MCMB – Infrastructure

The Medical Center for Molecular Biology (MCMB) is located in the old building of the institutes of the Faculty of Medicine on Vrazov trg 2. It has modern equipped laboratories for genomics, proteomics, cell cultures and functional genomic analyzes.

General Equipment

Laminary Flow Cabinet (Iskra Pio)

Responsible administrator:

Dubravka Germ

dubravka.germ@mf.uni-lj.si

Incubator Shaker C24 (New Brunswick Scientific)

Responsible administrator:

Dubravka Germ

dubravka.germ@mf.uni-lj.si

Rotation Shaker Cabinet GFL 7601

Responsible administrator:

Dubravka Germ

dubravka.germ@mf.uni-lj.si

Ultra Low Freezer -83⁰C MDF-U53V (Sanyo)

Responsible administrator:

Petra Hudler

petra.hudler@mf.uni-lj.si

Ultra Low Freezer -80⁰C MDF-534-PE (Panasonics)

Responsible administrator:

Pia Pužar Dominkuš

pia.puzar-dominkus@mf.uni-lj.si

Set of combined +4⁰C/-20⁰C storage cabinets

Responsible administrator:

Dubravka Germ

dubravka.germ@mf.uni-lj.si

Set of medium/high-temperature incubators/sterilizers

Responsible administrator:

Dubravka Germ

dubravka.germ@mf.uni-lj.si

Ultrapure water preparation system Pure Lab ELGA Flex 3

Responsible administrator:

Dubravka Germ

dubravka.germ@mf.uni-lj.si

Genomics

Nucleic Acids Isolation System Quickgene-810 (FUJIFILM):

Automated system for isolation of DNA/RNA from varied samples with high quality and high yield.

Responsible administrator:

Aleša Kristan

alesa.kristan@mf.uni-lj.si

Luminiscent Image Analyzer LAS 4000 (FUJIFILM):

Imaging system with six interchangeable light sources, a five position filter turret, and a 3.2-megapixel Sepper CCD imaging chip, enabling a range of applications including fluorescence, chemiluminescence, bioluminescence, and in vivo imaging.

Responsible administrators:

Ivana Jovčevska

ivana.jovcevska@mf.uni-lj.si

Aleša Kristan

alesa.kristan@mf.uni-lj.si

Eppendorf Centrifuge 5417 C

Responsible administrator:

Dubravka Germ

dubravka.germ@mf.uni-lj.si

Eppendorf Centrifuge 5810 R

Responsible administrator:

Dubravka Germ

dubravka.germ@mf.uni-lj.si

Proteomics

ÄKTA Pure Protein Purification System

Responsible administrators:

Neja Šamec

neja.samec@mf.uni-lj.si

Ivana Jovčevska

ivana.jovcevska@mf.uni-lj.si

Tailored and reliable chromatography system for fast purification of proteins, peptides, and nucleic acids from microgram to tens of gram levels of target product.

Applications supported:

Affinity chromatography
size exclusion chromatography (SEC, also known as gel filtration)
ion exchange chromatography
hydrophobic interaction chromatography
reversed phase chromatography (RPC).

IEF Multiphor II

Multiphor™ II electrophoresis system is a versatile modular system for horizontal electrophoresis, isoelectric focusing, 2-D electrophoresis and electrophoretic transfer.

Responsible administrator:

Nataša Debeljak

natasa.debeljak@mf.uni-lj.si

2D Eletrophoresis System Ettan DALT six

Semi-Dry Transfer Cell TRANS-BLOT SD (Biorad)

Responsible administrator:

Nataša Debeljak

natasa.debeljak@mf.uni-lj.si

High resolution Melt Software 2.0

PCR

Real-time Q-PCR Vii A7 (Applied Biosystms)

Responsible administrators:

Pia Pužar Dominkuš

pia.puzar-dominkus@mf.uni-lj.si

Katarina Kouter

katarina.kouter@mf.uni-lj.si

Gradient Thermal Cycler Simple-Amp (Applied Biosytems)

Responsible administrator:

Petra Hudler

petra.hudler@mf.uni-lj.si

Gene-Amp PCR System 2700 (Applied Biosystems)

Responsible administrator:

Petra Hudler

petra.hudler@mf.uni-lj.si

Gene-Amp PCR System 9700 (Applied Biosystems)

Responsible administrator:

Petra Hudler

petra.hudler@mf.uni-lj.si

Mastercycler X50s (Eppendorf)

Responsible administrators:

Alja Videtič Paska

alja.videtic@mf.uni-lj.si

Katarina Kouter

katarina.kouter@mf.uni-lj.si

Eppendorf Centrifuge 5418R

Responsible administrator:

Dubravka Germ

dubravka.germ@mf.uni-lj.si

Molecular Biology – Functional Genomics Analytics

Pipetting Robot epMotion 5075 (Eppendorf)

Capacity: 12 ANSI/SBS microplate positions
Maximum pipetting accuracy from 1 to 1,000 µL
Compatible with tubes (0.5 mL to 50 mL) and microplates with up to 384 wells

Responsible administrator:

Petra Hudler

petra.hudler@mf.uni-lj.si

Applications:

Serial dilutions
Distributing reagents
Sample transfer from individual tubes to plates
Reformatting plates
Media change and other cell culture applications
Sample pooling
Real-time PCR set-up
Cell-based assay set-up
Immunoassay set-up
Setting concentrations or volumes

Synergy H4 (BioTek)

BioTek™ Synergy™ H4 Hybrid Modular System for characterization of optical properties of biologicl molecules.

Responsible administrator:

Alja Zottel

alja.zottel@mf.uni-lj.si

Detection modes:

Fluorescence Intensity
Time-Resolved Fluorescence
Fluorescence Polarization
AlphaScreen™/AlphaLISA
Luminescence
UV-Visible absorbance
FRET
TR-FRET
BRET
well area scanning and spectral scanning.

Microplate Strip Washer Elx50/8MF

Responsible administrator:

Dubravka Germ

dubravka.germ@mf.uni-lj.si

Cell Culture Laboratory

Two Laminary Flow Cabinets

New Brunswick Galaxy® 170 R CO<sub>2</sub> Incubator Series from Eppendorf

CO2 incubator Heracell 150L CO2 incubator

Eppendorf Galaxy 170R

Lab Centrifuge MPW 260

Liquid nitrogen storage Dewar

Cell Counter

Water Bath GFL 1003

Combined Refrigerator / Freezer

Binocular Optical Microscope

Responsible Cell Culture Laboratory Admininstrators:

Mirjana Liović

mirjana.liovic@mf.uni-lj.si

Neja Šamec

neja.samec@mf.uni-lj.si

Sandra Ropret – for CO2 incubator Eppendorf Galaxy 170R

sandra.ropret@mf.uni-lj.si

Additional, advanced microscopy equipment is available at the Institute of Biophysics at the Faculty of Medicine UL: IBF MF

EQUIPMENT RESERVATION

Equipment Reservation Calendar

Additional research facilities and equipment are located at the Institute of Biochemistry of the Faculty of Medicine UL (IBK MF) and at the Centre for Functional Genomics and Biochips (CFGBC).