In 1993 MCMB started collaboration with the Institute of Pathophysiology (IP) of the Faculty of Medicine in Ljubljana on molecular genetic analysis of acetylcholinesterase (AchE) gene and its expression in skeletal muscle.
Research projects:
- J3-0074 Expression and action of cholinergic components; national basic research project, Z. Grubič, 1/7/1998―30/6/2001.
- J1―8762 Molecular biology and genetics in biomedical research; national basic research project, R. Komel, 1/1/1997―31/12/1999.
- J3-7920 Transcriptional and posttranscriptional regulation of biosynthesis and function of cholinergic components in mammalian organisms; national basic research project, Z. Grubič, 1/1/1996―30/6/1998.
Faculty partner:
Institute of Patophysiology, Faculty of Medicine, University of Ljubljana (Prof. Zoran Grubič, M.D.).
Research translation:
During training and education of PhD students MCMB assisted in starting independent molecular biology laboratory at the Institute of Patophysiology of the Faculty of Medicine in Ljubljana where research on AchE genetic expression and its physiological impact continued under Prof. Grubič’s supervision.
Researchers involved:
| Researcher | Position |
| Radovan Komel | Head of Department / Project supervisor |
| Zoran Grubič | Project leader |
| Katarina Zajc-Kreft | PhD student (IP) |
| Martina Brank | PhD student (IP) |
Education:
| Student | Thesis | Title | Year | Supervisors |
| Andreja Kavšek | Dipl | Experiments for isolation of cDNA probe for the gene encoding alfa-subunit of acetylcholine receptor in rat. | 1996 | Radovan Komel
Zoran Grubič |
| Katarina Zajc-Kreft | MSc | Comparison of radioactive and non-radioactive methods in quantitative determination of the acetylcholinesterase mRNA at the study of acetylcholinesterase synthesis regulation in skeletal muscle. | 1995 | Zoran Grubič Radovan Komel |
Selected publications
- Miš K. et al. (2005): Expression and distribution of acetylcholinesterase among the cellular components of the neuromuscular junction formed in human myotube in vitro. Chemico-Biological Interactions 157/158: 29-35.
- Zajc-Kreft K. et al. (2000): Nonradioactive northern blotting for the determination of acetylcholinesterase mRNA. Comparison to the radioactive technique. J. Physiol. – Pflügers Archiv 439(3, suppl.): R66-R67.
- Grubič Z. et al. (1999): Control levels of acetylcholinesterase expression in the mammalian skeletal muscle. Chemico-biological Interactions 119-20: 309-319.
- Brank M. et al. (1998): Biogenesis of acetylcholinesterase is impaired, although its mRNA level remains normal, in the gluccorticoid – treated rat skeletal muscle. J. Biochem. 251: 374-381.
- Grubič Z. et al. (1995): Myoblast fusion and innervation with rat motor nerve alter distribution of acetylcholinesterase and its mRNA in cultures of human muscle. Neuron 14(2): 317-327.
- Grubič Z., Komel R. (1993): Synthesis of the DNA probe for the determination of rat AChE mRNA. Chemico-biological Interactions 87(1-3): 245-248.